Inactivation of pea leaf phosphofructokinase by light and dithiothreitol.
نویسندگان
چکیده
When intact pea (Pisum sativum L.) plants are illuminated, the glycolytic enzyme phosphofructokinase is inactivated. In crude extracts the enzyme is inhibited by dithiothreitol. It would seem that this cytoplasmic enzyme, like glucose-6-P dehydrogenase, is light-inactivated when the enzymes of photosynthetic carbon metabolism are light-activated.
منابع مشابه
Light modulation of phosphofructokinase in pea leaf chloroplasts.
Chloroplastic phosphofructokinase, phosphorylase, phosphoglucomutase, and phosphoglucoisomerase in peas are light inactivated. The effect of light on phosphofructokinase is mimicked by dithiothreitol. DCMU, arsenite, and sulfite inhibit light modulation of the enzyme. No effect of light inactivation on the K(m) (fructose-6-P) or pH optima of phosphofructokinase was observed.
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ورودعنوان ژورنال:
- Plant physiology
دوره 55 2 شماره
صفحات -
تاریخ انتشار 1975